Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rios-Avila, L.; Prince, S.; Wijeratne, S.; Zempleni, J.
    A 96-well plate assay for high-throughput analysis of holocarboxylase synthetase activity (2011), Clin. Chim. Acta, 412, 735-739.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
6.3.4.10 analysis 96-well plate assay for high-throughput analysis of holocarboxylase synthetase activity by biotinylation of the polypeptide p67, which comprises the 67 C-terminal amino acids in human propionyl-CoA carboxylase, including the biotin-binding site lysine-669, using IRDye-streptavidin and infrared spectroscopy. The minimal concentration of recombinant HCS that can be detected by this assay is less than 1.08 nmol/l. Jurkat cells contain 0.14 U of HCS activity [in micromol of biotinylated p67 formed/(nmol/l HCS h)] in 400 microg of total protein Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.10 HCS fused to glutathione S-transferase, S-tag, and both N-terminal and C-terminal 6x His-tags Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.10 4.1
-
 p67 pH 8.0, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.10 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.10 JURKAT cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.10 ATP + biotin + p67 comprises the 67 C-terminal amino acids in human propionyl-CoA carboxylase, including the biotin-binding site lysine-669 Homo sapiens AMP + diphosphate + biotinyl-p67
-
 ?